Car vibrates at 2000 rpm--40mph

1990 MB 300 SELwith 250K miles. After starting in Drive, car vibrates significantly at 40 MPH. Increasing speed lessens vibs but increases its frequency. Tires,balance and alignment

ok. With car on lift still vibrates in gear at 2000 rpm

Tires,balance and alignment ok.

How do you know they are OK?

Had tires balanced and aligned initially.